Amplification of RNA transcripts using terminal continuation
نویسندگان
چکیده
منابع مشابه
Terminal continuation (TC) RNA amplification without second strand synthesis.
Terminal continuation (TC) RNA amplification was developed originally to reproducibly and inexpensively amplify RNA. The TC RNA amplification method has been improved further by obviating second strand DNA synthesis, a cost-effective protocol that takes less time to perform with fewer manipulations required for RNA amplification. Results demonstrate that TC RNA amplification without second stra...
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A novel methodology named terminal continuation (TC) RNA amplification has been developed to amplify RNA from minute amounts of starting material. Utility of the TC RNA amplification method is demonstrated with two new modifications including obviating the need for second strand synthesis, and purifying the amplification template using column filtration prior to in vitro transcription (IVT). Us...
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ژورنال
عنوان ژورنال: Laboratory Investigation
سال: 2003
ISSN: 0023-6837,1530-0307
DOI: 10.1038/labinvest.3700005